Methods in Molecular Biology and Protein Chemistry

Subtitle:
Cloning and Characterization of an Enterotoxin Subunit
Author:
Brenda D. Spangler
Format:
Softcover

Was:R1,302.95
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eBucks:eB10950

United States of America

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Methods in Molecular Biology and Protein Chemistry

Short description

Written in the style of a discovery-based guide, "Methods in Molecular Biology" enables students to master molecular biology and biochemistry techniques as an integrated part of a research project.

Long description

The enormous advances made in molecular biology have allowed scientists to manipulate DNA with relative ease. This means that molecular biology has become a widely-used tool for answering scientific questions that may be quite unrelated to genetics and cell biology. This new book focuses on the most important techniques needed by undergraduate and post-graduate students when undertaking research in this area. Written in an accessible style and adopting a 'discovery' approach, to encourage the students to explore and experiment for them selves, this book will be invaluable to novices required and interested in learning these techniques. Students will evaluate the effect of genetic engineering through the practical application of wet biochemistry techniques and prepare mutations that can be evaluated by other classes. Coverage will include computer tutorials for sequencing and genomics and will possibly include some coverage of molecular modelling too. Guidelines on searching the primary literature, oral reports, laboratory notebooks record keeping and report writing will either be included within appendices at the back of the book, or on a supplementary web site linked to the book.

Product details

Publisher:
John Wiley & Sons
ISBN:
9780470843604
Publication date:
June 2002
Additional format:
Paper Text
Length:
277mm
Width:
213mm
Thickness:
15mm
Weight:
626g
Pages:
209
Illustrations:
Illustrated
Readership:
Tertiary education; Professional & scholarly
Illustrated:
Illustrated

Table of contents

  • Preface
  • Introduction and Background
  • Part
  • Molecular Biology
  • Experiment Instructions for Pipetman Use
  • Experiment Preparation of Plasmid Template DNA
  • Experiment (a)Estimation of Plasmid DNA Concentration (b)PCR Amplification of LTB Gene from Plasmid DNA
  • Experiment (a)Agarose Gel Electrophoresis (b)Recovery of PCR Product
  • Experiment (a)Restriction Digest of LTB Insert (b)Plate Preparation
  • Experiment (a)Purification of Digested Insert and Vector by Agarose Gel Eletrophoresis (b)Recovery of Digested Insert and Vector
  • Experiment (a)Determination of DNA Concentrations (b)Ligation of Insert into Vector
  • Experiment Transformation of Host Cells by Construct
  • Experiment (a)Colony
  • pick PCR to Check for Presence of Insert (b)Streak Plates with Potential Constructs (c)Inoculate Cultures/Run PCR
  • Experiment (a)Agarose Gel Electrophoresis to Verify Presence of Insert (b)Purification of the pETLTB using a Plasmid Miniprep Kit
  • Experiment (a)DNA Concentration Determination (b)Ethanol Precipitation of Plasmid DNA (c)Sequencing Reaction
  • Experiment (a)Purification of Extension Reaction Products (b)Sequencing Gel Demonstration
  • Experiment (a)Analysis of Sequencing Data (b)Verification of Insert Sequence
  • Experiment (a)Gene Expression (b)Transformation into Expression Host
  • Experiment Induction of LTB Gene Expression
  • Determination of Time to Maximal Expression
  • Part
  • Protein Chemistry
  • Experiment (a)SDS
  • PAGE of Induction Time Course (b)Transfer of Protein to Membrane for Western Blot
  • Experiment Visualization of Western Blot
  • Experiment Large
  • scale Culture Preparation
  • Experiment Isolation of LTB by Affinity Chromatography
  • Experiment (a)SDS
  • PAGE of Column Fractions (b)Immunoblot to Verify Presence of LTB
  • Experiment Protein Concentration and Protein Crystallization
  • Experiment Setting up an ELISA Microtiter Plate to Assay a Panel of Ganlioside Ligands
  • Experiment ELISA of a Panel of Ganglioside Ligands
  • Experiment Protein Structure Determination
  • X
  • ray Diffraction Techniques
  • Experiment Characterization of a Protein Crystal using X
  • Ray Diffractemetry (optional)
  • Experiment Primer Design
  • Index

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Methods in Molecular Biology and Protein Chemistry

Methods in Molecular Biology and Protein Chemistry

Was:R1,302.95
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eBucks:eB10950
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