Short description
Each chapter in this text identifies a problem in neurobiology, showing how the solution of that problem will advance knowledge in the field.
Long description
Each chapter identifies a problem in neurobiology, showing how the solution of that problem will advance knowledge in the field. The approaches taken to solve the problem are clearly explained, providing sufficient detail for users to readily reproduce the technique and its results. In keeping with the goal of the series, each technique is designed to work, even the first time it is used.
Review
...an informative book that I would find a useful addition to my book shelf. I suspect that my copy will soon be stolen by a graduate student - always a good test of a book. - Trends in Neurosciences
Table of contents
- Separation of Large DNA Molecules by Pulsed-Field Gel Electrophoresis. The Isolation of Genomic DNA from Invertebrates. Analysis of Mitochondrial DNA Mutations. The Design and Use of Oligonucleotides. The Use of Degenerate Oligonucleotides for Polymerase Chain-Reaction-Based Isolation of Related DNA Sequences. Isolation of Photoreceptor Cell-Specific MEKA cDNA by Differential Hybridization. Generation of Isoform-Specific Antisera from Cloned cDNAs: Application to Multiple Forms of the Na/K-ATPase Expressed in Rat Brain. Determination of Transcriptional Initiation Sites and Their Usage in the Nervous System. Mapping of Trans-Acting Factor Binding in the Nervous System. In Situ Hybridization to Brain Tissue Sections Using Labeled Single-Strand Complementary RNA Probes. In Situ Hybridization Histochemistry Using Alkaline Phosphatase- Labeled Oligodeoxynucleotide Probe. The Identification of Neuropeptide Gene Regulatory Elements in Transgenic Mice. Expression of Exogenous Ion Channels and Neurotransmitter Receptors in RNA- Injected Xenopus Oocytes. Analysis of Insulin and Insulin-Like Growth Factor-I Receptors in Neural Tissues. Use of Affinity Chromatography in Purification of A1 Adenosine Receptors from Rat Brain Membranes. Purification and Structure of L-Type Calcium Channels. Purification and Reconstitution of the Ryanodine-Sensitive Ca2+ Release Channel Complex from Muscle Sarcoplasmic Reticulum. Identification of a Ligand-Gated Ion Channel by Photoaffinity Labeling and Microsequencing. Voltage-Gated Ion Channels: Electrophysiological Approaches. An Electrophysiological Approach to the Regulation of Neuronal Voltage-Activated Calcium Channels by Guanine Nucleotide Binding Proteins. Index.